Toluidine blue dye as a breast localization marker

Primary inducible fluorescence in secretory granules of tumor cells supravitally stained by a toluidine blue dye.

In a toluidine blue dye (British Drug Houses, batch no. 651880), we report a purple chromato­ graphic fraction (Rf—0,55) with a spectral absorption peak at 622 m/u, which has the property to induce immediate bright yellow fluorescence (primary inducible fluorescence) in cytoplasmic gra­ nules of tumor cells. The cells are unfixed, supravital stained and examined by fluorescence micro­ scopy. Th...

Significance of methylene blue dye for localization biopsy of nonpalpable breast lesions.

BACKGROUND AND OBJECTIVE With advances in mammographic equipment and techniques, more and more nonpalpable breast lesions have been detected. This study was to investigate the application of methylene blue dye for localized biopsy to diagnose nonpalpable breast lesions. METHODS In total 138 patients with suspicious malignant, nonpalpable breast lesions between August 2002 and October 2006 wer...

Blue dye, blue skin.

In vitro lethal photosensitization of S. mutans using methylene blue and toluidine blue O as photosensitizers.

The purpose of this in vitro study was to evaluate the antimicrobial effect of photodynamic therapy on Streptococcus mutans (A TCC 25175) suspensions, using a red laser for one minute in combination with toluidine blue O (TBO) or methylene blue (MB). Both photosensitizers were used in three concentrations (25, 10 and 5 mg/L). The activity ofphotosensitizers and laser irradiation were tested sep...

Toluidine blue O and methylene blue as endothelial redox probes in the intact lung.

There is increasing evidence that the redox activities of the pulmonary endothelial surface may have important implications for the function of both lungs and blood. Because of the inherent complexity of intact organs, it can be difficult to study these activities in situ. Given the availability of appropriate indicator probes, the multiple-indicator dilution (MID) method is one approach for de...